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Leaves are unfortunately quite complicated. There are different 'zones' of a leaf that have different characteristics. So when one wants to make comparisons between leaves one needs to make certain that the same areas/zones of different leaves are being compared.
In a very simplified description a small young developing leaf will not necessarily look the same as various parts of an older developmentally mature leaf but still be normal. Near where a leaf is attached to the crown the cells will be small - that is a zone where cell divisions are occurring. Further along the leaf there will be an elongation zone where cells elongate to as much as seven or more times their original length and then further still along the leaf there will be a maturation zone.
As well, the different types of cells do not develop in the same locations of a leaf at the same times. Stomata take some time to develop and appear. A very young leaf may not have any visible stomata in a particular zone because they have not yet started to develop while in the older leaf all the stomata may have finished developing (and appearing) in all parts of the leaf.
Although some of these developmental phases are known for corn I don't know if any of them are known for daylilies.
So the comparison of cell sizes and presence or absence of stomata, etc., would need to be done on a colchicine treated fan using a leaf of a particular age/size/location in the developmental sequence versus a leaf of the same age/size/location in the developmental sequence from a non-colchicine treated fan. The comparisons would need to be done with sections from the same zones (locations) on the two different leaves.
When making comparisons only one known factor can be different if one wants to be reasonably confident about the cause of any differences seen.
Read More...4 minutes ago
Now to shift gears a little, on February 18th I trimmed some of the outer leaves off the other conversion attempts. I did this because I wanted to get air circulating to the newest leaves and meristem to try to prevent rot (as recommended by Bill Waldrop). When I did this, here is what I observed…
[File Attachment: IMG_2236.jpg]
[File Attachment: IMG_2225.jpg]
Most plants looked like the first image (IMG_2236), but two of them looked like the second image. Since I assumed this was the first stage of rot, I cut those to look more like this…
[File Attachment: IMG_2226.jpg]
I did remove more on the plant shown so there wouldn’t be any brown spots. If it was some kind of bacteria or fungus, I didn’t want it to spread. ***Although this isn’t proof of anything (association, not causation) I wanted to note that all of the plants were kept unwatered through this point, except for the two that showed rot. I then let these continue to grow. Here is what they looked like on February 22nd (This is the same image I used in an earlier post)…
[File Attachment: IMG_2266_2014-03-12.jpg]
Then, on February 24th and 25th, I applied water to all of the plants dropwise using a pipette. Each day I only applied a Dixie cup worth of water split between all of the plants. I watered in the morning to avoid having wet soil through the night, and I kept the water away from the crown. I did this to try to avoid further rot.
On March 1st I started to notice more rot. Unfortunately I forgot to take a picture before I started trimming, but here is what they looked like before I removed it all (notice the blackened leaf tips on the lower left plant).
[File Attachment: IMG_2270.jpg]
Most of the rot damage looked like what was shown in IMG_2226, but some had the blackened appearance. I have some thoughts about this, but I’m going to save that for a later post.
After seeing this rot I applied Daconil fungicide on March 2nd. On March 4th and 5th I applied more water in the same manner I did before. Since I didn’t see anymore rot, I gave each plant a full Dixie Cup worth of water on March 7th and 8th. Here is what they look like now…
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To go back again and answer some questions…
Is the outward growth where the arrow is pointing?
I'm not convinced that outward growth is an indication of successful conversion. It might or might not be an indication that colchicine has affected some cells in the leaves. Unfortunately colchicine affects microtubules and microtubules are involved in cell structure and shape. Microtubules are also involved in cell division.
How would outward growth be caused by a successful leaf conversion? How would it manage to seem uniform?
By outward growth I meant more of an overall horizontal growth or swelling, which can be seen between IMG_2060 and IMG_2135. I wanted to avoid saying horizontal growth because I didn’t want anyone to think I was talking about the growth that results from a secondary meristem (a meristem that is responsible for the widening/secondary/horizontal growth in trees and other plants). Although this widening doesn’t mean there was conversion, it indicated to me that the chemical treatment likely reached and affected the leaves. I now have images that show the chemical treatment reached those inner leaves (not necessarily all the cells of the meristem) and show the change in the cell shape like you suggested might be happening.
If and when I get the time and space to do so, I would like to look more in-depth into some of the areas and questions that popped up during this process. Of course I would want to make sure I have a lot more plants to experiment with and I would want to use a less expensive plant that I don’t mind if I destroy.
Read More...18 hours 50 minutes ago
It’s been a while, but here’s an update on my conversion attempts.
To go back a little bit, here is what they looked like on February 17th…
[File Attachment: IMG_2143.jpg]
Then, although this was originally just supposed to be my first time doing meristem conversions, I strayed a little bit. To try to prove to myself that the treatments were working, I decided to remove the outer leaves on one of the conversions so I could look at the leaves under the microscope. Here is what it looked like (taken on Feb. 17th)…
[File Attachment: IMG_2193.jpg]
I would have removed more leaves, but it was an expensive plant and I didn’t want to kill it intentionally - since the original intent was to convert the plants.
To show what I found, I’m going to start with an image showing the surface of a normal, untreated daylily leaf (image taken Feb. 17th)
[File Attachment: IMG_2177.jpg]
Notice the elongated cells and the stomata. Now here is the surface of the leaf just outside the remaining leaf shown in IMG_2193 (a treated plant)...
[File Attachment: IMG_2188.jpg]
Notice the rounded shape of ALL cells, and how you can no longer distinguish the stomata from the other epidermal cells.
Read More...18 hours 58 minutes ago
Wow looks like both ATTAM and tetKI battled to dominate the flower. Looks like a bit of both... Looks to be potentially nice. Lots of teeth for sure, wonder how hot it was that day? My select toothy seedlings were super toothy on really hot days. I'm sure you had a very fun bloom season. I will be speaking at the April 5 PDS meeting with George Doorakian and Curtis Noel, should be a good time. I will also have a seedling slide show of 2013 bloom season. Keep up the good work dan.
Ps the crosses you sent me from the LA will probably bloom this summer
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One of my favorite pattern seedlings, it's an odd one!
Read More...3 days ago
Suzanne Warnsing updated their profile picture.3 days ago
Thanks Dan. I just added a few photos of some of my larger flowers to my 2012 seedling album - most aren't close up pictures. So far the one seedling you've already seen is my one impressive Monster seedling (or the only one I still grow). I hope to have a few more bloom for the first time this summer - including a few out of that one. I also have a list of about 30 crosses to make this coming summer, which will hopefully produce some of my largest seedlings yet. It's too bad that even if they work, which could be iffy working with such large flowers, they won't bloom until 2016 (at the earliest). But it gives me something to look forward to. Does anyone else keep a list of future crosses, or is this a sign I have a problem ?
You have some extremely impressive patterned seedlings Dan. I still remember some of those seedlings you showed at the local daylily meeting, plus during the winter I go back to an old Paul Lewis blog post now and again to check them out.
Read More...6 days ago