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Now to shift gears a little, on February 18th I trimmed some of the outer leaves off the other conversion attempts. I did this because I wanted to get air circulating to the newest leaves and meristem to try to prevent rot (as recommended by Bill Waldrop). When I did this, here is what I observed…
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Most plants looked like the first image (IMG_2236), but two of them looked like the second image. Since I assumed this was the first stage of rot, I cut those to look more like this…
[File Attachment: IMG_2226.jpg]
I did remove more on the plant shown so there wouldn’t be any brown spots. If it was some kind of bacteria or fungus, I didn’t want it to spread. ***Although this isn’t proof of anything (association, not causation) I wanted to note that all of the plants were kept unwatered through this point, except for the two that showed rot. I then let these continue to grow. Here is what they looked like on February 22nd (This is the same image I used in an earlier post)…
[File Attachment: IMG_2266_2014-03-12.jpg]
Then, on February 24th and 25th, I applied water to all of the plants dropwise using a pipette. Each day I only applied a Dixie cup worth of water split between all of the plants. I watered in the morning to avoid having wet soil through the night, and I kept the water away from the crown. I did this to try to avoid further rot.
On March 1st I started to notice more rot. Unfortunately I forgot to take a picture before I started trimming, but here is what they looked like before I removed it all (notice the blackened leaf tips on the lower left plant).
[File Attachment: IMG_2270.jpg]
Most of the rot damage looked like what was shown in IMG_2226, but some had the blackened appearance. I have some thoughts about this, but I’m going to save that for a later post.
After seeing this rot I applied Daconil fungicide on March 2nd. On March 4th and 5th I applied more water in the same manner I did before. Since I didn’t see anymore rot, I gave each plant a full Dixie Cup worth of water on March 7th and 8th. Here is what they look like now…
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To go back again and answer some questions…
Is the outward growth where the arrow is pointing?
I'm not convinced that outward growth is an indication of successful conversion. It might or might not be an indication that colchicine has affected some cells in the leaves. Unfortunately colchicine affects microtubules and microtubules are involved in cell structure and shape. Microtubules are also involved in cell division.
How would outward growth be caused by a successful leaf conversion? How would it manage to seem uniform?
By outward growth I meant more of an overall horizontal growth or swelling, which can be seen between IMG_2060 and IMG_2135. I wanted to avoid saying horizontal growth because I didn’t want anyone to think I was talking about the growth that results from a secondary meristem (a meristem that is responsible for the widening/secondary/horizontal growth in trees and other plants). Although this widening doesn’t mean there was conversion, it indicated to me that the chemical treatment likely reached and affected the leaves. I now have images that show the chemical treatment reached those inner leaves (not necessarily all the cells of the meristem) and show the change in the cell shape like you suggested might be happening.
If and when I get the time and space to do so, I would like to look more in-depth into some of the areas and questions that popped up during this process. Of course I would want to make sure I have a lot more plants to experiment with and I would want to use a less expensive plant that I don’t mind if I destroy.
Read More...17 hours 59 minutes ago
It’s been a while, but here’s an update on my conversion attempts.
To go back a little bit, here is what they looked like on February 17th…
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Then, although this was originally just supposed to be my first time doing meristem conversions, I strayed a little bit. To try to prove to myself that the treatments were working, I decided to remove the outer leaves on one of the conversions so I could look at the leaves under the microscope. Here is what it looked like (taken on Feb. 17th)…
[File Attachment: IMG_2193.jpg]
I would have removed more leaves, but it was an expensive plant and I didn’t want to kill it intentionally - since the original intent was to convert the plants.
To show what I found, I’m going to start with an image showing the surface of a normal, untreated daylily leaf (image taken Feb. 17th)
[File Attachment: IMG_2177.jpg]
Notice the elongated cells and the stomata. Now here is the surface of the leaf just outside the remaining leaf shown in IMG_2193 (a treated plant)...
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Notice the rounded shape of ALL cells, and how you can no longer distinguish the stomata from the other epidermal cells.
Read More...18 hours 7 minutes ago
Wow looks like both ATTAM and tetKI battled to dominate the flower. Looks like a bit of both... Looks to be potentially nice. Lots of teeth for sure, wonder how hot it was that day? My select toothy seedlings were super toothy on really hot days. I'm sure you had a very fun bloom season. I will be speaking at the April 5 PDS meeting with George Doorakian and Curtis Noel, should be a good time. I will also have a seedling slide show of 2013 bloom season. Keep up the good work dan.
Ps the crosses you sent me from the LA will probably bloom this summer
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One of my favorite pattern seedlings, it's an odd one!
Read More...3 days ago
Suzanne Warnsing updated their profile picture.3 days ago
Thanks Dan. I just added a few photos of some of my larger flowers to my 2012 seedling album - most aren't close up pictures. So far the one seedling you've already seen is my one impressive Monster seedling (or the only one I still grow). I hope to have a few more bloom for the first time this summer - including a few out of that one. I also have a list of about 30 crosses to make this coming summer, which will hopefully produce some of my largest seedlings yet. It's too bad that even if they work, which could be iffy working with such large flowers, they won't bloom until 2016 (at the earliest). But it gives me something to look forward to. Does anyone else keep a list of future crosses, or is this a sign I have a problem ?
You have some extremely impressive patterned seedlings Dan. I still remember some of those seedlings you showed at the local daylily meeting, plus during the winter I go back to an old Paul Lewis blog post now and again to check them out.
Read More...6 days ago
I have always liked going through previous seasons' images, especially when it's miserable and frozen outside. I have not taken tetTK to tetSS, and had not really considered it, actually. TK is a very weak northern plant, and SS is decent but not amazing in and of itself. Both have been shown to be great breeders. As I feel comfortable doing my own conversions, I'd rather name the diploid cross of the two, find a good plant there, then convert that. Neither of the conversions I had were pod fertile, in my experience, but both as very much so as dips, so I suspect a conversion could be made that was as well.
I have seen very few tet TK kids, but more from tet KALEIDOSCOPIC INTRIGUE. For teeth, I now I had some kids from tet TK pollen on TRULY ANGELIC, DESIRE OF NATIONS, and a toothy baby of mine from VERTICAL HORIZON X ENTWINED IN THE VINE). I have also gotten tet TK and tet KI onto ATTAM. One seedling from ATTAM x tet KI has bloomed, see attached. Based on this pic, maybe this will go well with toothy lines?
[File Attachment: Sdlg5-DR-ATTAMwithKI-dwhr-IDed.jpg]
Read More...6 days ago